甩尾測(cè)痛儀主要是用于測(cè)量大、小鼠尾巴部受紅外熱刺激的痛覺閾值。實(shí)驗(yàn)時(shí)，當(dāng)動(dòng)物感覺疼痛，尾巴會(huì)輕敲臺(tái)面，內(nèi)置傳感器會(huì)立刻檢測(cè)到，停止計(jì)時(shí)和關(guān)閉光源，即儀器自動(dòng)記錄反應(yīng)時(shí)間和光源強(qiáng)度。數(shù)據(jù)可通過U盤、USB數(shù)據(jù)線等導(dǎo)出。
甩尾測(cè)痛儀中有一可調(diào)強(qiáng)度的紅外光源，其紅外光主要是通過一拋物面反射鏡聚焦在實(shí)驗(yàn)動(dòng)物的尾巴上。實(shí)驗(yàn)時(shí)，操作人員將實(shí)驗(yàn)動(dòng)物置于儀器上，把動(dòng)物的尾巴放在紅外光源處，接受刺激。
儀器自動(dòng)記錄實(shí)驗(yàn)數(shù)據(jù)
精度高，避免人為因素引起的誤差
包含U盤和軟件
特征
優(yōu)點(diǎn)
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可獨(dú)立工作，也可連接電腦使用
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儀器工作臺(tái)表面無(wú)突出和遮擋物件
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操作方便，實(shí)驗(yàn)重復(fù)性好
規(guī)格:
命令：軟鍵和腳踏板
連接電腦：DELTA 9-pin連接頭，USB連線
數(shù)據(jù)讀?。阂壕э@示
電源：universal mains 85-264 VAC, 50-60Hz
打?。何⑿蜔崦舸蛴C(jī)（需另外購(gòu)買）
工作溫度：15° - 30° C
開始：紅外開關(guān)
聲級(jí)：< 70 dB
紅外強(qiáng)度：10-99級(jí)間可調(diào)
反應(yīng)時(shí)間：液晶屏顯示，分辨率為0.1s
校準(zhǔn)：紅外熱輻射計(jì)（需另外購(gòu)買）
截止時(shí)間：預(yù)置，15 - 60 s間
文獻(xiàn)
關(guān)于方法
- F.E. D’Amour & D.L. Smith: "A Method for Determining Loss of Pain Sensation." J. Pharmacol. Exp. Therap. 72: 74-79, 1941.
- D.C. Yeomans & H.K. Proudfit: “Characterization of the Foot Withdrawal Response to Noxious Radiant Heat in the Rat”Pain 59: 85-97, 1994.
涉及疼痛甩尾
- C. Dawson et alia: “ Dexmedetomidine Enhances Analgesic Action of Nitrous Oxide” Anesthesiology 100 (4): 894?904, 2004
- P. Tolu et alia: “ Effects of Long-Term Acetyl-L-carnitine Administation in Rats: I. Increased Dopamine Output in Mesocorticolimbic Areas and Protection Toward Acute Stress Exposure” Neuropsychopharmacol. 27 (3): 410-420, 2002
- R. Nadeson et alia: “ Potentiation by Ketamine of Fentanyl Antinociception. I. An Experimental Study in Rats Showing that Ketamine Administered by Non-Spinal Routes Targets Spinal Cord Antinociceptive Systems” Br. J. Anaesthesia 88 (5): 685?691, 2002
- L. Jasmin et alia: “ The NK1 Receptor mediates Both the Hyperalgesia and the Resistance to Morphine in Mice Lacking Noradrenaline” PNAS 99 (2): 1029?1034, 2002
- G.L. Fraser et alia: “ Antihyperalgesic Effects of Opioid Agonists in a Rat Model of Chronic Inflammation” Br. J. Pharmacol. 129: 1668?1672, 2000
- M. Xu et alia: “ Effects of Radolmidine, a Novel α2- Adrenergic Agonist Compared with Dexmedetomidine in Different Pain Models in the Rat” Anesthesiology 93 (2): 473?481, 2000
- A. K?ster et alia: “Targeted Disruption of the Orphanin Fq/Nociceptin Gene Increases Stress Susceptibility and Impairs Stress Adaptation In Mice” Neurobiology 96 (18): 10444-10449, 1999
- I. Sora et alia: “Opiate Receptor Knockout Mice Define μ Receptor Roles in Endogenous Nociceptive Responses and Morphine-Induced Analgesia” Neurobiology 94: 1544-1549, 1997
- C.T. Dourish et alia: "The Selective CCK-B Receptor Antagonist L-365,260 Enhances Morphine Analgesia and Prevents Morphine Tolerance in the Rat" Europ. J. Pharmacol. 176: 35-44, 1990
- P.W. Nance & J. Sawinok: "Substance P-Induced Long-Term Blockade of Spinal Adrenergic Analgesia: Reversal by Morphine and Naloxone" J. Pharmacol. Exp. Therap. Vol. 240, No. 3: 972-977,
